Nucleotide BLAST: Search nucleotide databases using a nucleotide query Program Selection Optimize for Optimize for Highly similar sequences (megablast) Optimize for More dissimilar sequences (discontiguous megablast) Optimize for Somewhat similar sequences (blastn) Choose a BLAST algorithm Help Search database core_nt using Megablast (Optimize for highly similar sequences) Show results in a new window Algorithm
BLAST+ executables — BLASTHelp documentation BLAST+ executables What are the next steps? Magic-BLAST IgBLAST SRPRISM Databases BLAST+ executables ¶ Do you have difficulties running high volume BLAST searches? Do you have proprietary sequence data to search and cannot use the NCBI BLAST web site? Do you have access to your own server? Do you have your own research pipeline? Have security or IP concerns about sending searches outside of
Nucleotide BLAST: Search nucleotide databases using a nucleotide query PSI-BLAST allows the user to build a PSSM (position-specific scoring matrix) using the results of the first BlastP run PHI-BLAST performs the search but limits alignments to those that match a pattern in the query DELTA-BLAST constructs a PSSM using the results of a Conserved Domain Database search and searches a sequence database
Query Input and database selection - BLAST Query Input and database selection ¶ The query sequence (s) to be used for a BLAST search should be pasted in the ‘Search’ text area BLAST accepts a number of different types of input and automatically determines the format or the input To allow this feature there are certain conventions required with regard to the input of identifiers (e g , accessions or gi’s) These are described
Welcome to BLAST Help — BLASTHelp documentation Welcome to BLAST Help ¶ Getting started ¶ Web BLAST quick start guide New BLAST databases New Guide to BLAST home and search pages Blast report description Blast topics Frequently Asked Questions Getting Help ¶ Write to the help desk Mailing list BLAST tutorials About BLAST ¶ NCBI Handbook The Statistics of Sequence Similarity Scores BLAST
Smart BLAST Smart Blast searches a protein query against the landmark database Enter Protein Query Sequence
Needleman-Wunsch alignment of two nucleotide sequences - BLAST Title: Core nucleotide BLAST database Description: The core nucleotide BLAST database consists of GenBank+EMBL+DDBJ+PDB+RefSeq sequences, but excludes EST, STS, GSS, WGS, TSA, patent sequences as well as phase 0, 1, and 2 HTGS sequences and most eukaryotic chromosome sequences The database is non-redundant
Frequently Asked Questions — BLASTHelp documentation The BLAST programs are command line programs that run BLAST searches against local, downloaded copies of the NCBI BLAST databases, or against custom databases formatted for BLAST The programs can handle either a single large file with multiple FASTA query sequences, or you can create a script to send multiple files one at a time
BLAST Highlights BLAST Assembled Genomes contains links to genomic BLAST pages for common organisms, and a link to a complete list of available organism genome BLAST pages Basic BLAST contains links to BLAST forms for the traditional set of databases (e g , nr, est, etc ) Choose the link for the search type you want